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  文件名稱: ECACC General Cell Collection:10032309 PEO4
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ECACC General Cell Collection:10032309 PEO4


ECACC General Cell Collection:10032309 PEO4
一般細胞收集細節
ECACC一般細胞收集:PEO4
受供於:
歐洲認證細胞培養物保藏中心(ECACC)
文化類型:
細胞係
采集:
ECACC General Collection
產品編號:
10032309
細胞係名稱:
PEO 4
引文指導:
如果使用這種文化產生科學出版物,則應在出版物中引用:PEO4(ECACC 10032309)
關鍵詞:
人卵巢癌; 雌激素受體陽性
細胞係描述:
PEO4 is an adherent cell line derived from a malignant effusion from the peritoneal
ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient
previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO4was
collected after clinical resistance developed to chemotherapy. PEO4 is tumourigenic in
immunologically-deprived CBA mice. PEO4 exhibits good growth in semi-solid medium
(agar). PEO4 is from the same patient as the PEO1 and PEO6 cell lines (ECACC
catalogue numbers 10032308 & 10032310).This cell line is one of nine from the PE
ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian
cancer, isolated from various malignant sites, and at various treatment stages) available
at ECACC which provides a model system for research into the mechanism of oestrogen
action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity
of oestrogen protagonists.
種類:
人的
原產地組織:
子房
單元格類型:
上皮細胞; 均勻多邊形細胞的島嶼。
成長模式:
追隨者
DNA簡介:
STR-PCR數據:
釉原蛋白:X
CSF1PO:10,12
D13S317:10
D16S539:9
D5S818:11,12
D7S820:10
THO1:9.3
TPOX:9,11
vWA:15,16
核型:
對於模態染色體數目,參見Langdon SP等。(1988)癌症研究11月1日; 48(21):
6166-72。2019/9/17
ECACC General Cell Collection
10032309 PEO4
生物安全信息:
Unless specified
otherwise, at the European
C
ollection of Authenticated Cell Cultures
(ECACC) we routinely handle all of our cell lines at containment level 2 in accordance
with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the
responsibility of the end user to ensure that their facilities comply with biosesafety
regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare
premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine:
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10 cells/cm using
0.25% trypsin or trypsin/EDTA; 5% CO ; 37°C. Doubling time approximately 36 hours.
Cultures should be media changed every 2-3 days if not splitting.
Culture Medium:
RPMI 1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum
(FBS).
Depositor:
Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street
London EC1V 4AD
Originator:
Yes
Country:
UK
References:
Langdon SP et.al. (1988) Characterization and properties of nine human ovarian
adenocarcinoma cell lines. Cancer Research Nov 1; 48(21):6166-72. PMID: 3167863.
Additional Bibliography:
Langdon SP et.al. (1990) Oestrogen receptor expression and the effects of oestrogen
and tamoxifen on the growth of human ovarian carcinoma cell lines. British Journal of
Cancer Aug; 62(2):213-6. PMID: 2386737.
Patents:
None specified by Depositor
Release Conditions:
Yes - commercial organisations are required to complete the 'Cell Line Release
Authorisation for Research Use in Commercial Organisations' release conditions form.
Product Images
10032309_PEO4_Growth Profile
Note: Links open in a new window
Product Documents
10032309_PEO4_Images
Passage numbers explained
CRT Cell Line Release Authorisation for Research Use in Commercial Organisations
Note: Links open in a new window
Related Products
You might be interested in the following related products:
ECACC General Cell Collection - PEO1
ECACC General Cell Collection - PEO6
ECACC General Cell Collection - PEO14
ECACC General Cell Collection - PEO16
ECACC General Cell Collection - PEO23
ECACC General Cell Collection - TO14
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to
ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for
any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the
4 2
22019/9/17
ECACC General Cell Collection
10032309 PEO4
cell culture and not for any specific property of
the cell line, therefore furth
er
references should be obtained
regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections
does not guarantee the passage number stated will be the passage number received by the customer.
Culture Collections提供的文化僅供研究之用。關於細胞係的商業用途的詢問涉及細胞係的保藏者。一些細胞係具有
額外的特殊釋放條件,例如在供應細胞係之前需要由潛在接受者完成材料轉移協議。請查看供應條款和條件以獲取
更多信息。
可用格式
冷凍
生長
DNA-5μg(100ng /μl)
RNA-5μg(100ng /μl)
cDNA-20μl
快速鏈接
ECACC - 細胞係
NCTC - 細菌
NCPV - 病毒
NCPF - 真菌
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ECACC General Cell Collection:10032309 PEO4


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